Evaluation of indirect diagnostic tests and PBMC expression of innate immune genes in subclinical mastitis in dairy cows

The aim of this study was to estimate the prevalence subclinical mastitis (SCM), its innate immunity and compare efficacy indirect diagnostic tests [California test (CMT), differential electrical conductivity (DEC), absolute EC, enzyme based color detection method (Tanucheck kit) brothymol blue strip (BTB) tests] milk somatic cell count (SCC) using 200×103 cells/ml as a cut off value in Deoni (N=27) HF crossbred (N=32) cows. Cumulative SCM 38% 63% cows, respectively on SCC basis (N=215). Breed, udder health status interaction had significant effect level. overall sensitivity specificity above were 38 99%; 52 72%; 69%; 76 31%, 1 100%, respectively. mRNA expression serum amyloid A (SAA), IL-1β TNF- α genes significantly up-regulated while, TNF-α gene down-regulated PBMC affected It is concluded that, DEC more suitable detect SAA strongly related